论文部分内容阅读
[目的]探讨甲状腺乳头状癌(PTC)中mir-221表达和BRAF基因状态的相关性和临床意义。[方法]采用RNA核酸印迹(Northern blot)法检测43例PTC癌组织及其癌旁正常组织中mir-221表达,直接测序法检测PTC癌组织中BRAF基因状态,分析mir-221表达和BRAF基因状态的相关性及其与临床病理特征的关系。[结果]mir-221在PTC癌组织中的表达显著高于配对正常组织(中位值:0.855vs0.549,P<0.001);mir-221高表达与临床分期(P=0.002)及淋巴结转移(P=0.042)有关。BRAF基因在PTC癌组织中的突变率为53.49%(23/43)。突变型BRAF的甲状腺癌患者组mir-221表达显著高于野生型BRAF患者组(P=0.024)。[结论]mir-221与BRAF基因在PTC的发生、发展过程中起重要作用,mir-221表达升高与BRAF基因突变有一定相关性。
[Objective] To investigate the correlation and clinical significance of mir-221 expression and BRAF gene status in papillary thyroid carcinoma (PTC). [Methods] The expression of mir-221 in 43 cases of PTC and its adjacent normal tissues was detected by Northern blot. The status of BRAF gene in PTC tissues was detected by direct sequencing. The expression of mir-221 and the expression of BRAF gene Correlation and its relationship with clinicopathological features. [Results] The expression of mir-221 in PTC tissues was significantly higher than that in paired normal tissues (median: 0.855 vs 0.549, P <0.001). The high expression of mir-221 was correlated with clinical stage (P = 0.002) and lymph node metastasis (P = 0.042). The mutation rate of BRAF gene in PTC tissues was 53.49% (23/43). Mir-221 expression was significantly higher in patients with thyroid cancer than in patients with wild-type BRAF (P = 0.024). [Conclusion] The mir-221 and BRAF genes play an important role in the development and progression of PTC. The increased expression of mir-221 may be related to the mutation of BRAF gene.