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目的:观察原花青素(PC)对心肌梗死(MI)大鼠炎性浸润及其对肿瘤坏死因子-α(TNF-α)表达的影响。方法:结扎大鼠左冠状动脉前降支构建大鼠MI模型,存活大鼠随即分为MI组和PC组,另设假手术组(Sham组),各组按观察时相2周和4周再分为:MI 2周和4周,PC 2周和4周,sham 2周和4周;组织病理学观察术后2周及4周各组心肌组织病理结构;逆转录聚合酶联反应(RT-PCR)检测TNF-α的mRNA表达,Westernblotting及免疫组织化学检测TNF-α蛋白表达。结果:MI后2周及4周:①与Sham组比较,MI组和PC组心肌组织结构显著异常;与MI组比较,PC组心肌结构明显改善。②与Sham组比较,MI组和PC组TNF-α在基因和蛋白水平上显著增加,差异有统计学意义(P<0.01);与MI组比较,PC组TNF-α在基因或蛋白水平上表达显著减少,差异有统计学意义(P<0.01)。结论:PC能够减轻大鼠MI后的炎性浸润,下调TNF-α表达。
Objective: To observe the effect of proanthocyanidins (PC) on inflammatory infiltration and the expression of tumor necrosis factor-α (TNF-α) in rats with myocardial infarction (MI). Methods: The rat model of MI was established by ligation of the left anterior descending coronary artery of rats. The surviving rats were divided into MI group and PC group. Sham group (sham group) The rats were divided into 2 weeks and 4 weeks of MI, 2 weeks and 4 weeks of PC, 2 weeks and 4 weeks of sham. Histopathological examination was performed to observe the pathological changes of myocardial tissue in each group at 2 weeks and 4 weeks after operation. Reverse transcription polymerase chain reaction The mRNA expression of TNF-α was detected by RT-PCR. The protein expression of TNF-α was detected by Western blotting and immunohistochemistry. Results: Two weeks and four weeks after MI: ①Compared with Sham group, the myocardial structure of MI group and PC group were significantly abnormal. Compared with MI group, the myocardial structure of PC group was significantly improved. ②Compared with Sham group, the levels of TNF-αin MI group and PC group were significantly increased at gene and protein levels (P <0.01); Compared with MI group, The expression was significantly reduced, the difference was statistically significant (P <0.01). Conclusion: PC can reduce inflammatory infiltration and down-regulate the expression of TNF-α after MI in rats.