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目的:观察中药胃肠安对胃癌细胞的丙二醛(MDA)、超氧化物岐化酶(SOD)、乳酸脱氢酶(LDH)及核转录因子NF-κB表达的影响,并探讨胃肠安在胃癌微环境中氧化应激及炎症活化的作用。方法:获取胃肠安药物血清,以胃癌细胞(SGC-7901细胞株)为研究对象,设对照组和胃肠安处理组,检测胃癌细胞SOD、MDA、LDH的水平,Western Blot检测胃癌细胞NF-κB蛋白的表达。结果:与对照组相比,胃肠安处理组中胃癌细胞MDA、LDH降低,而SOD水平升高;对照组胃癌细胞NF-κB蛋白相对表达量为(0.68±0.12),与对照组相比,胃肠安处理组胃癌细胞NF-κB蛋白相对表达量(0.36±0.04)降低。结论:胃肠安可通过抑制胃癌细胞的氧化应激和炎症,改变肿瘤细胞生存微环境,最终可能发挥抑制肿瘤生长的作用,其具体效应有待深入研究。
Objective: To observe the effect of weiguanan on the expression of malondialdehyde (MDA), superoxide dismutase (SOD), lactate dehydrogenase (LDH) and NF-κB in gastric cancer cells, Ann in gastric cancer microenvironment oxidative stress and inflammatory activation. Methods: The gastric cancer cells (SGC-7901 cell line) were obtained from the gastric cancer cells (SGC-7901). The control group and the gastrointestinal safety treatment group were used to detect the levels of SOD, MDA and LDH in gastric cancer cells. -κB protein expression. Results: Compared with the control group, the gastric cancer cell MDA and LDH decreased and the SOD level increased in the treatment group. The relative expression level of NF-κB protein in the gastric cancer cells in the control group was (0.68 ± 0.12), compared with the control group , And the relative expression of NF-κB protein in gastric cancer cells treated with weiguan (0.36 ± 0.04) decreased. CONCLUSION: Gastrointestinal Ankang can change the survival microenvironment of gastric cancer cells by inhibiting the oxidative stress and inflammation of gastric cancer cells, and eventually may play an inhibitory role in tumor growth. The specific effect needs further study.