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稻瘿蚊是亚洲稻区主要害虫,采用抗虫品种进行防治是最理想的方法。1993～1995年,广东省农科院与国际水稻研究所有关专家紧密合作,对能抗华南4个稻瘿蚊生物型的品种多抗1作进一步抗性遗传分析,确认多抗1对中国稻瘿蚊生物型1和4的抗性受显性单基因控制,这个基因暂定名为GM—6(t)。以多抗1×丰银占1组合的F3代160个家系作基因标记,据DNA库分离个体分析(BSA)原理,用随机扩增多态性DNA(RAPD)标记物OPM6(1.4kb),首次成功地标记了这个抗性基因。随后多态性扩增产物经~(32)p标记,用作探针,检测另一个参考作图群体IR64×Azucena,将这个抗性基因定位在水稻第4条染色体上,位于RG214和RG163两个DNA限制性片段长度多态性(RFLP)标记之间。应用这些分子标记辅助选择有可能不必通过稻瘿蚊的直接筛选,快速准确地选育抗稻瘿蚊品种或进行抗性基因累加。
Rice gall midge is a major pest in the Asian rice region, and the use of insect-resistant varieties is the best way to control. From 1993 to 1995, the Guangdong Provincial Academy of Agricultural Sciences and the International Rice Research Institute in close cooperation with experts, resistant to four southern China rice gall midge biotypes of multi-resistance genetic analysis for further resistance 1, confirmed that the multi-resistance of a pair of Chinese rice The resistance of Aedes albopictus biotypes 1 and 4 was controlled by a dominant single gene, tentatively named GM-6 (t). A total of 160 F3 lines were genotyped by a combination of polyclonal anti-1 × HSBC-1 combinations. According to the principle of DNA library separation and individual analysis (BSA), random amplified polymorphic DNA (RAPD) markers OPM6 (1.4kb) The first successful marker of this resistance gene. Subsequently, the polymorphic amplified product was labeled with ~ (32) p and used as a probe to detect another IR64 × Azucena mapping locus on the fourth chromosome of rice, located at RG214 and RG163 DNA restriction fragment length polymorphism (RFLP) marker between. With these molecular marker-assisted selection, it is possible to rapidly and accurately select and breed anti-gall midge mosquito species or accumulate resistance genes without the need of direct screening of rice gall midge.