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[目的]研究葛根素对酒精性肝纤维化(HF)的影响。[方法]21只Wistar大鼠随机分为3组,每组7只,对照组用50%葡萄糖20 ml/(kg.d)灌胃,模型组用40%乙醇8 g/(kg.d)灌胃,葛根素组用40%乙醇8 g/(kg.d)灌胃加葛根素10 mg/kg腹腔注射,3组均给药16周。处死大鼠取血清及肝脏,通过光镜观察肝组织病理变化并进行纤维化积分,放免法测定血清透明质酸(HA)、Ⅲ型前胶原(PCⅢ)及层黏连蛋白(LN)。[结果]葛根素明显改善HF程度,与对照组比较差异有统计学意义(P<0.05),与模型组比较,血清HA、PCⅢ及LN显著降低(P<0.05)。[结论]葛根素具有较强的抗HF作用。
[Objective] To study the effect of puerarin on alcoholic liver fibrosis (HF). [Method] Twenty-one Wistar rats were randomly divided into three groups with 7 rats in each group. The control group was orally administered with 50% glucose 20 ml / (kg · d), and the model group was treated with 40% ethanol 8 g / (kg · d) The rats in the puerarin group were treated with puerarin (10 mg / kg) by intraperitoneal injection of 40% ethanol (8 g / (kg · d)) for 16 weeks. The rats were sacrificed and the serum and liver were taken out. The pathological changes of liver tissue were observed under light microscope and the fibrosis scores were determined. Serum hyaluronic acid (HA), PC Ⅲ and laminin (LN) were detected by radioimmunoassay. [Results] Puerarin significantly improved the level of HF, compared with the control group, the difference was statistically significant (P <0.05), compared with the model group, serum HA, PC Ⅲ and LN were significantly lower (P <0.05). [Conclusion] Puerarin has strong anti-HF effect.