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为探讨脂质过氧化对血管内皮细胞源生长因子的影响，以氢过氧化枯烯作用于培养的牛主动脉内皮细胞后，收集内皮细胞条件培养基，并用肝素亲和层析法处理，分组测定其对3T3细胞3H-胸腺嘧啶核苷掺入率的影响，并用放射免疫法测定各脂质过氧化作用组内皮细胞培养基中内皮素的含量。结果发现．氢过氧化枯烯使培养的内皮细胞的脂过氧化物蓄积增加，且内皮细胞条件培养基对3T3细胞的DNA合成有促进作用．对Swiss3T3细胞的促增殖活性主要存在于非肝素结合部分。氢过氧化枯烯作用后的内皮细胞的内皮素分泌水平比对照组增加。由此推测脂质过氧化作用引起的内皮细胞对平滑肌细胞的促增殖活性可能与内皮素等非肝素结合的分子有关。
In order to investigate the effect of lipid peroxidation on the growth factor of vascular endothelial cells, cortisone hydroperoxide was used to culture endothelial cells of bovine aorta, then the conditioned medium of endothelial cells was collected and treated with heparin affinity chromatography The 3H-thymidine incorporation rate of 3T3 cells was measured and the content of endothelin in endothelial cell culture medium was determined by radioimmunoassay. turn out. Cumene hydroperoxide increases the lipid peroxidation of cultured endothelial cells, and endothelial cell conditioned media promotes the DNA synthesis of 3T3 cells. Proliferative activity on Swiss3T3 cells is mainly present in the non-heparin binding moiety. The endothelin secretion level of endothelial cells after the action of cumene hydroperoxide was higher than that of the control group. It is speculated that lipid peroxidation-induced endothelial cell proliferation of smooth muscle cells and proliferative activity may be related to non-heparin molecules such as endothelin.