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采用逆转录聚合酶链反应（PCR）技术自SD大鼠肝细胞中克隆出全长1125个bP的编码主要组织相容性抗原Ⅰ类复合体分子（MHC－Ⅰ）cDNA。经限制性内切酶图谱分析证实后，定向插人表达载体pBV220，并筛选出带有插人片断的阳性克隆。为研究在原核或真核表达系统中的表达，及其在抗原识别，免疫应答中的作用奠定了基础。
The cDNA of major histocompatibility antigen class Ⅰ complex (MHC-Ⅰ) with a total length of 1125bP was cloned from SD rat hepatocytes by reverse transcription polymerase chain reaction (PCR). After confirmed by restriction endonuclease mapping, the expression vector pBV220 was inserted into the vector and the positive clones with inserted fragments were screened out. It laid the foundation for studying the expression in prokaryotic or eukaryotic expression system and its role in antigen recognition and immune response.