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CaSn基因是在辣椒(Capsicum annuum)‘Santaka’中发现的新型抗菌肽Snakin家族基因。通过RT-PCR,将CaSn基因从辣椒中分离并构建到pBI-121植物表达载体,通过农杆菌EHA105介导转化白肋烟(Nicotiana tabacum‘White burley’),筛选到10个独立的转基因株系,并对T1代植株进行抗南方根结线虫(Meloidogyne incognita)鉴定及靶标基因表达分析。结果表明CaSn已转入白肋烟,能够进行超量表达,并且转CaSn基因白肋烟上的根结数量比转空载体对照和非转基因对照减少70%,同时不同转基因烟草株系间对南方根结线虫的抗性也存在着一定差别。通过试验证明了辣椒中的抗菌肽基因CaSn具有抗南方根结线虫作用,对于植物抗线虫资源的挖掘及利用提供了重要理论依据。
The CaSn gene is a novel antimicrobial peptide Snakin family found in Capsicum annuum ’Santaka’. The CaSn gene was isolated from pepper and constructed into pBI-121 plant expression vector by RT-PCR. Agrobacterium tumefaciens (WB) and Burley (Burley) were transformed by Agrobacterium tumefaciens EHA105. Ten independent transgenic lines The T1 plants were identified against Meloidogyne incognita and their target genes were analyzed. The results showed that CaSn had been transferred into burley tobacco and over-expressed, and the number of root junctions on Cauliflower tobacco leaves decreased by 70% compared with the control and non-transgenic control. Meanwhile, Root knot nematode resistance there are some differences. The experiment proved that CaSn, an antimicrobial peptide gene in pepper, has the anti-root-knot nematode effect and provides an important theoretical basis for the excavation and utilization of plant-resistant nematode resources.