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目的:建立抗柯萨奇B病毒性心肌炎胶囊(K-CoxB-JN)定性和定量检测方法,为其提供质量控制手段。方法:采用薄层色谱法对方中西洋参、麦冬、莱菔子、王不留行4味药物进行定性鉴别;采用HPLC-ELSD法测定西洋参中人参皂苷Rg1,Re,Rb1含量。以Agilent ZORBAX XDB C18(4.6 mm×250 mm,5μm)为色谱柱,流动相乙腈-0.2%甲酸(梯度洗脱),柱温为室温;ELSD参数:漂移管温度50℃,载气流速2.0 L.min-1,增益1。结果:薄层色谱专属性强,分离度好,图谱斑点清晰,阴性对照无干扰;人参皂苷Rg1,Re,Rb1质量分别在0.75～3.75,4.5～30,6～40μg对数值与相应峰面积对数值呈良好的线性关系(r>0.999),平均回收率分别为97.10%(RSD 3.96%),95.15%(RSD 2.21%),95.60%(RSD 3.00%)。结论:该方法简便、准确、重复性好且无干扰,可用于K-CoxB-JN中主要药材的鉴别以及人参皂苷Rg1,Re,Rb1的含量控制。
Objective: To establish a qualitative and quantitative detection method of K-CoxB-JN capsules and provide quality control measures. Methods: TLC, HPLC, ELSD were used to determine the contents of ginsenoside Rg1, Re and Rb1 in Panax quinquefolius L. The mobile phase was acetonitrile-0.2% formic acid (gradient elution) with a column of Agilent ZORBAX XDB C18 (4.6 mm × 250 mm, 5 μm) at room temperature. ELSD parameters: drift tube temperature 50 ℃, carrier gas flow 2.0 L .min-1, gain 1. The results showed that TLC was of high specificity, good resolution, clear spots on the spectrum and no interference on the negative control. The ginsenosides Rg1, Re and Rb1 were between 0.75 ~ 3.75,4.5 ~ 30,6 ~ 40μg and the corresponding peak area pairs The values showed a good linear relationship (r> 0.999). The average recoveries were 97.10% (RSD 3.96%), 95.15% (RSD 2.21%) and 95.60% (RSD 3.00%), respectively. Conclusion: The method is simple, accurate, reproducible and non-interfering and can be used for the identification of the main herbs in K-CoxB-JN and the content control of ginsenosides Rg1, Re and Rb1.