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目的 建立山东地区的肝癌细胞系,并研究其生物学特性.方法 将临床获取肝癌标本用组织块法进行体外培养,观察肝癌细胞的形态学、核型、增殖动力学和AFP功能.结果 肝癌细胞系(HCC-9810)具有典型的上皮细胞特征,染色体数目22～116,细胞倍增时间约20.4h.结论提示HCC-9810是一株新建肝癌细胞系,可作为进一步肝癌研究的模型.
Objective To establish a liver cancer cell line in Shandong Province and study its biological characteristics. Methods Clinical specimens of liver cancer were cultured in vitro using a tissue block method to observe the morphology, karyotype, proliferation kinetics, and AFP function of the hepatoma cells. The lineage (HCC-9810) has typical epithelial cell characteristics with a chromosome number of 22 to 116 and a cell doubling time of about 20.4 hours. Conclusion HCC-9810 is a newly established hepatoma cell line that can be used as a model for further studies of liver cancer.