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利用肾上腺素(EPI)来诱导近江牡蛎眼点幼虫产生单体牡蛎苗。首先对眼点幼虫占80%~90%幼虫群体,采用不同浓度的EPI(1.0×10-5 M、5×10-5 M、1×10-4 M、5×10-4 M、1.0×10-3 M、5×10-3 M)持续处理3h;其次对眼点幼虫占20%~30%、50%~60%和80%~90%幼虫群体,在最佳EPI浓度下,持续诱导3h;然后对眼点幼虫占80%~90%幼虫群体,在最佳EPI浓度下,持续诱导1h、2h、3h、4h、5h、6h;同时进行了相同密度下,单体牡蛎和固着牡蛎的生长变化的比较。实验结果表明:当眼点幼虫出现80%~90%时,EPI浓度为10-4 M时,持续诱导3h,得到了最高的不固着变态率,为(60.0±4.8)%;相同密度下,附着牡蛎苗生长快于单体牡蛎苗。
Epinephrine (EPI) was used to induce monoecious oyster shoots in Oyster ’s eye spot larvae. First of all, 80% ~ 90% of larvae were exposed to different concentrations of EPI (1.0 × 10-5 M, 5 × 10-5 M, 1 × 10-4 M, 5 × 10-4 M, 1.0 × 10-3 M, 5 × 10-3 M) for 3 h, followed by 20% -30%, 50% -60% and 80% -90% larvae of the larvae of the eyed tree at the best EPI concentration And then induced the larvae of the larvae at 80% ~ 90% of the larval population for 1h, 2h, 3h, 4h, 5h, 6h at the optimal EPI concentration. At the same time, Comparison of growth changes in oysters. The experimental results showed that when the spot larvae appeared 80% ~ 90%, the highest rate of non-fixation metamorphosis was obtained (60.0 ± 4.8%) when the EPI concentration was 10-4 M for 3 hours. Under the same density, Attached oyster seedlings grow faster than single oyster seedlings.