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目的 观察大鼠胚胎神经上皮细胞同种脑移植后的存活及生长分化状况。 方法 孕 12d大鼠剖腹取胚胎 ,剥离神经管 ,胰酶消化后获取神经管壁上的神经上皮细胞 ,然后植入其父体侧脑室中。分别于移植后 10d、14d、2 1d、2 8d灌注取脑 ,用HE染色及神经元特异烯醇化酶 (NSE)、胶质纤维酸性蛋白 (GFAP)免疫组织化学方法检测胚胎神经上皮细胞移植后的存活及分化状况。 结果 神经上皮细胞侧脑室移植后多贴附于脑室壁形成细胞团块 ,有的随脑脊液循环至第三脑室生长。随时间延长移植物增大。HE染色见脑室内有成团的移植细胞 ;免疫组织化学染色显示移植细胞团内既有NSE 免疫阳性细胞 ,也有GFAP 免疫阳性细胞。移植物周围多为GFAP 免疫阳性细胞。 结论 胚胎神经上皮细胞侧脑室移植后能够贴附脑室壁存活 ,并能分化为神经元和神经胶质细胞
Objective To observe the survival, growth and differentiation of rat embryonic neuroepithelial allografts after brain transplantation. Methods Pregnant 12-day-old rats were killed by embryo dissection and stripped of neural tube. After digestion with trypsin, neuroepithelial cells on the neural tube wall were obtained and then implanted into the lateral ventricle of their father. The brains were harvested at 10d, 14d, 21d and 28d after transplantation, respectively. After HE staining and neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP) immunohistochemical staining, Survival and differentiation status. Results The neuroepithelial cells were attached to the ventricular wall and formed clumps of cells after transplantation. Some of them circulated to the third ventricle with the growth of cerebrospinal fluid. Increased graft over time. Hematoxylin and eosin (HE staining) showed that there were cells in the brain that were agglomerated. Immunohistochemical staining showed that there were both NSE-positive cells and GFAP-positive cells in the transplanted cells. GFAP immunopositive cells mostly around the graft. Conclusion Embryonic neuroepithelial cells can be attached to the ventricular wall after transplantation and can differentiate into neurons and glial cells