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为了研究水杨酸对重金属胁迫下植物幼苗的缓解效应,以玉米叶片的叶绿素、游离脯氨酸、可溶性蛋白质量比和丙二醛(MDA)含量,以及过氧化物酶(POD)与超氧化物酶(SOD)活性为指标,采用水培法研究了不同质量浓度水杨酸对150 mg/L Pb2+胁迫下玉米幼苗生理特性的影响。结果表明,经30 mg/L水杨酸缓解后受迫玉米幼苗的根长、株高和根系活性明显增加,叶片的叶绿素、游离脯氨酸与可溶性蛋白质量比均明显提高,叶片的SOD与POD活性均高于对照组和各处理组,且叶片的MDA含量显著降低。外源水杨酸可通过正向调控植物物质与能量代谢,以及保护酶系统活性来维持细胞膜的稳定性,并有效减缓膜脂过氧化进程,缓解了Pb2+胁迫对玉米幼苗的毒害。
In order to study the alleviating effect of salicylic acid on plant seedlings under heavy metal stress, the effects of salicylic acid on chlorophyll content, free proline content, soluble protein content and malondialdehyde (MDA) content in maize leaves, (SOD) activity as an index. The effects of different concentrations of salicylic acid on the physiological characteristics of maize seedlings under 150 mg / L Pb2 + stress were studied by hydroponics. The results showed that the root length, plant height and root activity of maize seedlings under stress of 30 mg / L salicylic acid were significantly increased, and the ratios of chlorophyll, free proline to soluble protein in leaves were significantly increased. The contents of SOD and POD activity were higher than the control group and each treatment group, and the leaf MDA content decreased significantly. Exogenous salicylic acid can maintain cell membrane stability through positive regulation of plant matter and energy metabolism, as well as protect the activity of the enzyme system, and effectively reduce the membrane lipid peroxidation process, alleviating the toxicity of Pb2 + to corn seedlings.