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目的比较介观荧光体层成像(MEFT)和EPRI-照明反射成像(EPRI)来定量评估小鼠肿瘤的大小。方法在注射后14d用MEFT、EPRI、超声(US)和微型计算机体层扫描(μCT)测量结肠癌细胞肿瘤移植绿/红色荧光蛋白(GFP/RFP)的表达(n=6)。MEFT和EPRI彼此之间,并且与US及μCT相关(对照法)。肿瘤体积体外测量用GFP和RFP荧光成像与体内测量进行比较。结果 MEFT与US和μCT之间具有高的相关性和一致性(MEFT/US:GFP,r2=0.96;RFP,r2=0.97,两者P<0.05;MEFT/μCT:GFP,r2=0.93;RFP,r2=0.90;两者P<0.05)。此外,体内MEFT数据和体外成像结果具有高度相关性和一致性(GFP:r2=0.96;RFP,r2=0.99;两者P<0.05)。相比而言,EPRI显著地高估了肿瘤体积(P<0.05),虽然它与US和μCT有显著的相关性(EPRI/US:GFP,r2=0.95;RFP,r2=0.94;两者P<0.05。EPRI/μCT:GFP,r2=0.86;RFP,r2=0.86;两者P<0.05)。结论应用MEFT的荧光分布重构可提供非常精确的三维(3D)肿瘤体积数据,比EPRI更准确。因此,MEFT是在高空间分辨率上定量评估表浅肿瘤荧光分布非常合适的技术。
Objective To quantitatively evaluate the size of mouse tumors by comparing mesoscopic fluorescent layer imaging (MEFT) and EPRI-illumination reflectance imaging (EPRI). Methods Colon cancer cell tumor-transplanted green / red fluorescent protein (GFP / RFP) expression (n = 6) was measured by MEFT, EPRI, US, and micro-computed tomography (μCT) 14 days after injection. MEFT and EPRI are related to each other and to US and μCT (control method). In vitro tumor volume measurements were compared with in vivo measurements using GFP and RFP fluorescence imaging. Results MEFT was highly correlated and consistent with US and μCT (MEFT / US: GFP, r2 = 0.96; RFP, r2 = 0.97, both P <0.05; MEFT / μCT: GFP, r2 = , r2 = 0.90; both P <0.05). In addition, in vivo MEFT data were highly correlated and consistent with in vitro imaging results (GFP: r2 = 0.96; RFP, r2 = 0.99; both P <0.05). In contrast, EPRI significantly overestimated tumor volume (P <0.05), although it had a significant association with US and μCT (EPRI / US: GFP, r2 = 0.95; RFP, r2 = 0.94; <0.05.EPRI / μCT: GFP, r2 = 0.86; RFP, r2 = 0.86; both P <0.05). Conclusions The fluorescence distribution reconstruction using MEFT can provide very accurate three-dimensional (3D) tumor volume data, which is more accurate than EPRI. Therefore, MEFT is a very suitable technique for quantitative assessment of superficial tumor fluorescence distribution at high spatial resolution.