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[Objective] This study aimed to investigate the four Gastrodia elata Bl. variants at the DNA level. [Method] Primers with good reproducibility, abundant polymorphism and high resolution were selected from the 35 random primers with RAPD marker technology and the amplification results were analyzed. [Result] By using RAPD technology, DNA fingerprints of four Gastrodia elata Bl. variants with abundant polymorphism, good reproducibility and high resolution was successfully constructed; to be specific, each Gastrodia elata Bl. variant has its unique fingerprint with primers S29 and S38; DNA bands of the Gastrodia elata Bl. variants vary significantly, which is contributive to distinguish the four Gastrodia elata Bl. variants. [Conclusion] This study provided an effective means for the identification and protection of Gastrodia elata germplasms with intraspecific variations.
[Objective] This study aimed to investigate the four Gastrodia elata Bl. [Method] Primers with good reproducibility, abundant polymorphism and high resolution were selected from the 35 random primers with RAPD marker technology and the amplification results were analyzed . [Result] By using RAPD technology, DNA fingerprints of four Gastrodia elata Bl. Variants with abundant polymorphism, good reproducibility and high resolution was successfully constructed; to be specific, each Gastrodia elata Bl. Variant has its unique fingerprint with primers S29 and S38 ; DNA bands of the Gastrodia elata Bl. Variants of significantly, which is contributive to distinguish the four Gastrodia elata Bl. Variants. [Conclusion] This study provides an effective means for the identification and protection of Gastrodia elata germplasms with intraspecific variations.