采用免疫组化ＡＢＣ－ＧＤＮ及计算机辅助图象分析技术，研究猫的交感神经、舌咽神经切断对中耳粘膜的Ｐ物质（ＳＰ）、神经肽Ｙ（ＮＰＹ）免疫反应及中耳积液的影响。结果发现：交感神经切断后，中耳粘膜ＮＰＹ免疫反应明显减少，中耳积液量和ＮＰＹ免疫反应光密度值呈负相关。切断舌咽神经对中耳积液及ＳＰ、ＮＰＹ免疫反应无影响。并就中耳粘膜ＳＰ、ＮＰＹ的起源及其含量变化的意义进行了讨论。 Immunohistochemical ABC-GDN and computer-aided image analysis techniques were used to study the cat’s sympathetic nerve and glossopharyngeal nerve cut off the substance P (SP), neuropeptide Y (NPY) immune response and middle ear effusion influences. The results showed that after sympathectomy, the NPY immunoreactivity in the middle ear mucosa was significantly reduced, and the amount of effusion in the middle ear was negatively correlated with the NPY immunoreaction optical density. Cut off the glossopharyngeal nerve on the middle ear effusion and SP, NPY immune response no effect. The significance of the origin and content of SP and NPY in middle ear mucosa was also discussed.