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目的上皮钙黏着蛋白(E-cadherin)的编码基因CDH1是重要的肿瘤抑制基因,本研究探讨CDH1基因-160(C→A)多态性在福建地区胃癌人群中的分布及其与福建地区胃癌发病风险的相关性。方法采用聚合酶链反应-变性高效液相色谱分析方法对102例胃癌患者和101名正常对照者进行CDH1基因-160(C→A)多态的基因型分析,比较基因型分布和发病风险的关系;危险度OR及95%CI应用非条件Logistic回归分析计算。结果CDH1基因-160(C→A)多态的CC、CA、AA基因型在病例组中的分布频率分别为58(56.9%),38(37.3%),6(5.9%);在对照组的分布频率分别为55(54.5%),41(40.6%),5(5%);两组间分布的差异无统计学意义(P>0.05)。AA基因型没有显著性地提高或降低胃癌的发病危险(OR=1·12;95%CI:0.32~3.95);携带A等位基因与胃癌的临床病理特征也无关联性。结论CDH1基因-160(C→A)多态性可能与福建地区中国人群胃癌发生的遗传易感性无关。
Objective To investigate the distribution of CDH1 gene-160 (C → A) polymorphism in gastric cancer patients in Fujian Province and its relationship with gastric cancer in Fujian Province Correlation of risk of onset. Methods The genotypes of CDH1 gene -160 (C → A) polymorphism in 102 gastric cancer patients and 101 normal controls were analyzed by polymerase chain reaction-denaturing high performance liquid chromatography (RP-HPLC). The genotype distribution and risk of Relationship; Risk OR and 95% CI were calculated using unconditional logistic regression analysis. Results The frequencies of CC, CA and AA genotypes of CDH1 gene -160 (C → A) polymorphism were 58 (56.9%), 38 (37.3%) and 6 The distribution frequency was 55 (54.5%), 41 (40.6%) and 5 (5%) respectively. There was no significant difference between the two groups (P> 0.05). AA genotype did not significantly increase or decrease the risk of gastric cancer (OR = 1.12; 95% CI: 0.32-3.95). There was no correlation between the A allele and the clinicopathological features of gastric cancer. Conclusion The -160 (C → A) polymorphism of CDH1 gene may not be related to the genetic susceptibility to gastric cancer in Chinese population in Fujian Province.