论文部分内容阅读
为了探讨自体角朊细胞诱导免疫抑制的机制,我们利用已建立的MELR体外增殖系统,采用三色标记流式细胞术及定量PCR方法检测了自体角朊细胞共信号分子B7-H1和HLA II类分子的表达情况,观察其对角朊细胞所诱导的免疫抑制的影响。结果发现,自体而非异体角朊细胞可有效抑制MELR。在抑制系统中检测到B7-H1和II类分子高表达于自体角朊细胞表面。单抗封阻B7-H1,可逆转自体角朊细胞诱导的免疫抑制作用。结论:自体角朊细胞通过B7-H1介导对异种混合淋巴细胞增殖的抑制作用。
To explore the mechanism of autologous keratinocyte-induced immunosuppression, we used the established MELR in vitro proliferation system to detect the co-signaling molecules B7-H1 and HLA class II of autologous keratinocytes using the three-color flow cytometry and quantitative PCR Molecular expression, observe its keratinocyte-induced immune suppression. As a result, it was found that autologous, non-allogeneic keratinocytes effectively inhibited MELR. High levels of B7-H1 and class II molecules were detected on the surface of autologous keratinocytes in the suppressor system. Monoclonal antibody blocking B7-H1 reverses the immunosuppressive effects induced by autologous keratinocytes. Conclusion: The inhibition of proliferation of heterogeneous mixed lymphocytes mediated by autologous keratinocytes through B7-H1.