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①目的 制备胆囊收缩素（ Ｃ Ｃ Ｋ）ｃ Ｄ Ｎ Ａ 探针，并以此探针检测遗传性听源性癫痫易感大鼠癫痫发作后脑内 Ｃ Ｃ Ｋｍ Ｒ Ｎ Ａ 表达。②方法 将含０．５ｋｂｐ Ｒａｔ Ｃ Ｃ Ｋｍ Ｒ Ｎ Ａ 的质粒 Ｐ Ｕ Ｃ１３ 扩增、回收和纯化，用随机引物标记法，得到地高辛精（ Ｄｉｇ）标记 Ｃ Ｃ Ｋｃ Ｄ Ｎ Ａ 探针。用原位杂交技术，对癫痫发作后大鼠海马和大脑皮层 Ｃ Ｃ Ｋｍ Ｒ Ｎ Ａ 表达进行研究。③结果 Ｃ Ｃ Ｋｃ Ｄ Ｎ Ａ 探针标记效率为５０ｍ ｇ／ Ｌ；遗传性听源性癫痫易感大鼠癫痫发作后脑内 Ｃ Ｃ Ｋｍ Ｒ Ｎ Ａ 表达显著增加。④结论 本实验制备的 Ｄｉｇ Ｃ Ｃ Ｋｃ Ｄ Ｎ Ａ 探针，具有较好的特异性和灵敏度；原位杂交实验证实， Ｃ Ｃ Ｋ 参与了癫痫发作过程。
Objective To prepare the C C K m R N A probe for detection of seizures in hereditary auditory epilepsy rats. ② Methods Plasmid P U C13 containing 0.5kbp Rat C CK R N A was amplified, recovered and purified. Digitophoridine (Dig) labeled C C Kc D N A probe. In situ hybridization technique was used to study the expression of C C Km RN A in hippocampus and cerebral cortex of rats after epileptic seizure. Results The efficiency of C Kc D N A probe labeling was 50 m g / L. The expression of C C Km R N A in brains of epileptic seizures increased significantly in hereditary auditory epilepsy rats. ④ Conclusion Dig C K C D N A probe prepared in this experiment has good specificity and sensitivity; in situ hybridization experiments confirmed that C C K involved in the process of seizures.