目前,细胞蜡块技术已经在临床工作中广泛应用。尽管多数胸腔积液样本可以通过直接离心得到大量细胞沉淀,但是,仍有一部分样本由于阳性细胞量少,必须放入薄层液基细胞保存液(TCT保存液)中进行制片。经过该固定液处理的细胞不能够凝聚成团,离心后难以取出细胞沉淀。曾经有人采用蛋清~[1]或者琼脂~[2]凝胶作为细胞粘附基质,但是,该方法需要额外的材料。也有人采用细胞离心管~[3]技术,但 Currently, cell wax block technology has been widely used in clinical work. Although most pleural effusion samples can be pelleted by direct centrifugation, there are still some samples that have to be put into thin-layer liquid-based cell preservation solution (TCT preservation solution) due to the small number of positive cells. The cells treated with the fixative solution can not aggregate into clusters, and it is difficult to remove the cell pellet after centrifugation. Some people used egg white ~ [1] or agar ~ [2] gel as a cell adhesion matrix, however, this method requires additional material. Some people use cell centrifuge tube ~ [3] technology, however