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本文采用间接免疫荧光技术研究了矽肺大鼠和汉防己甲素(简称汉甲素)治疗矽肺的大鼠肺中Ⅰ型胶原的变化。Ⅰ型胶原是肺间质的重要成分。在发生矽肺纤维化时,肺泡间隔中的Ⅰ型胶原含量明显地高于正常的肺泡间隔。在矽结节中,可见到发射绿色荧光的Ⅰ型胶原纤维束排列致密,表明Ⅰ型胶原参与了矽肺纤维化。用汉甲素治疗后,矽肺纤维化受到抑制,胶原纤维松解、断裂,互相缠绕,说明汉甲素可减低胶原纤维的聚合程度,并对胶原有一定的降解作用。汉甲素治疗的大鼠矽肺中存在大量大细胞,大细胞胞浆内可见发射绿色荧光的Ⅰ型胶原,可能为大细胞所吞噬从而加速胶原的分解。
In this paper, the indirect immunofluorescence technique was used to study the changes of type I collagen in the lungs of silicosis rats and tetrandrine (abbreviated as anacardium) in the treatment of silicosis. Type I collagen is an important component of the lung interstitium. In the occurrence of pneumoconiosis fibrosis, the type I collagen content in the alveolar septum is significantly higher than the normal alveolar septum. In the sacral tubercle, it was observed that the type I collagen fiber bundles emitting green fluorescence were densely arranged, indicating that type I collagen was involved in the silicosis fibrosis. After treatment with hanbarin, the fibrosis of the pneumoconiosis was inhibited, and the collagen fibers were released, broken and intertwined, indicating that tigral can reduce the degree of polymerization of the collagen fibers and have a certain degree of degradation of collagen. There are a large number of large cells in the squamous lung of rats treated with hanbarin. Type I collagen emitting green fluorescence can be seen in the cytoplasm of large cells, which may be phagocytosed by large cells to accelerate the decomposition of collagen.