论文部分内容阅读
目的:探讨微小染色体维持蛋白2(MCM2)在宫颈上皮内瘤变、宫颈癌中的表达及其与临床病理特征之间的关系。方法:收集2008～2009年承德医学院附属医院经病理证实的20例高度宫颈上皮内瘤变(HSIL)、40例宫颈鳞癌、20例正常宫颈组织,应用S-P免疫组化方法检测以上组织标本中MCM2的表达情况。结果:MCM2的阳性表达指数从正常宫颈鳞状上皮、HSIL到宫颈鳞癌逐渐升高(P<0.01),其在宫颈鳞癌中的阳性表达与肿瘤的分化程度及淋巴转移相关(P<0.01;P<0.05),而与患者的年龄、肿瘤的体积及临床分期无关。结论:MCM2能够反映宫颈鳞状上皮细胞增殖程度,可作为宫颈鳞癌新的肿瘤标记物应用于临床,以评估病变的恶性程度。
Objective: To investigate the expression of minichromosome maintenance protein 2 (MCM2) in cervical intraepithelial neoplasia and cervical cancer and its relationship with clinicopathological features. Methods: Twenty cases of high grade cervical intraepithelial neoplasia (HSIL), 40 cases of cervical squamous cell carcinoma and 20 cases of normal cervical tissue were collected from 2008 to 2009 in Chengde Medical College Affiliated Hospital. SP immunohistochemistry was used to detect the above tissue specimens In MCM2 expression. Results: The positive expression of MCM2 in cervical squamous cell carcinoma was significantly higher than that in normal cervical squamous cell carcinoma and HSIL cervical squamous cell carcinoma (P <0.01). The positive expression of MCM2 in cervical squamous cell carcinoma correlated with tumor differentiation and lymphatic metastasis (P <0.01) ; P <0.05), but not with the patient’s age, tumor size and clinical stage. Conclusion: MCM2 can reflect the degree of cervical squamous cell proliferation and can be used as a new tumor marker in cervical squamous cell carcinoma to evaluate the malignant degree of cervical lesions.