目的 :建立荧光分光光度法测定人血浆中左氧氟沙星浓度。方法 :以三氯乙酸沉淀蛋白 ,分离后加醋酸 -醋酸钠缓冲液 (p H4.0 )测荧光强度 ,激发波长 2 94nm,发射波长 496 nm。结果 :左氧氟沙星浓度在 0 .1～ 5 .0 μg· m l- 1范围内有良好的线性关系 ,回归方程为 Y=2 4.916 X- 1.2 97,r=0 .9994。平均回收率为 98.5 2 % ,RSD为 3.12 %。结论 :该方法简便、快速 Objective: To establish a spectrofluorimetric method for the determination of levofloxacin in human plasma. Methods: The protein was precipitated with trichloroacetic acid, and the fluorescence intensity was measured with acetate-sodium acetate buffer (p H4.0) after separation. The excitation wavelength was 2 94 nm and the emission wavelength was 496 nm. Results: The levofloxacin concentration ranged from 0.1 to 5.0 μg · ml-1. The regression equation was Y = 2 4.916 X- 1.2 97, r = 0.9994. The average recovery was 98.5 2% with a RSD of 3.12%. Conclusion: This method is simple and fast