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目的研究HIV-1CRF01-AE亚型毒株与HIV传播及流行的关系,为预防控制艾滋病提供科学依据。方法分三个时段检测贵州省多地区190份HIV-1毒株样本,用套式PCR扩增其Env基因和测序分析,将实验结果与贵州省艾滋病流行现状进行相关分析。结果检出B,B’,E,C亚型及CRF07-BC,CRF08-BC和CRF01-AE三种新的流行性重组亚型毒株,流行性重组亚型毒株是造成贵州省艾滋病流行的因素之一,其中CRF01-AE毒株与性传播、16～50岁年龄段高度相关。结论 CRF01-AE亚型毒株是贵州省艾滋病传播与流行的主要亚型毒株;16～50岁年龄段是CRF01-AE亚型毒株通过性传播艾滋病的主体人群;CRF01-AE亚型毒株引起的艾滋病流行,具有传播范围广、流行时间长、控制难度大等特点。
Objective To study the relationship between HIV-1 CRF01-AE subtypes and HIV transmission and epidemic and to provide a scientific basis for the prevention and control of AIDS. Methods Totally 190 samples of HIV-1 strains from different areas of Guizhou Province were detected in three periods. The Env gene was amplified by nested PCR and sequenced. The results were compared with the prevalence of HIV / AIDS in Guizhou Province. Results Three novel recombinant subtypes of B, B ’, E, C and CRF07-BC, CRF08-BC and CRF01-AE were detected. The epidemic recombinant subtype strains were the most common cause of HIV / AIDS in Guizhou Province One of the factors, including CRF01-AE strains and sexually transmitted, 16 to 50 age-group is highly correlated. Conclusion The CRF01-AE subtype is the main subtype of AIDS transmission and epidemic in Guizhou Province. The main population of CRF01-AE subtypes is the main body of transmission of AIDS among 16-50 age groups. CRF01-AE subtypes Strain caused by AIDS epidemic, with a wide range of spread, long popular, difficult to control and so on.