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本文评价了由肺炎球菌荚膜多糖(PS)和B群脑膜炎球菌外膜蛋白复合物(OMPC)组成的四价结合菌苗在灰鼠中耳炎(OM)模型中的免疫原性和灰鼠对同型和异型菌攻击的抵抗能力.作者将70只1～2岁、体重400～600g的灰鼠随机分成8组,34只接种菌苗,36只接种安慰剂.菌苗是由6B、14、19F和23F型肺炎球菌PS与OMPC偶联而成.灰鼠分别于0和4周接种2剂菌苗,剂量为2μgPS/kg.首剂免疫后8周,分别将0.1ml的6A、6B、19A或19F型肺炎球菌悬液(200～370CFU/ml)注入灰鼠中耳下鼓室泡内进行攻击,攻击后3、7和14天作耳镜和鼓室检查及中耳吸引术.每次免疫前(0天和第4周)、攻击前(第8周)和攻击后2周(第10周)心脏穿刺采血,用ELISA法检测抗6A、6B、19A和19F型PS特异性IgG抗体.结果显示,免疫前灰鼠血清中未测得抗4型PS特异性抗体.免疫后,菌苗组抗6A、6B、19A和19F型PS特异性抗体显著增高,安慰剂组则无显著变化.在以6B或6A型菌
This article evaluated the immunogenicity of tetrabasic binding bacterins composed of pneumococcal capsular polysaccharide (PS) and meningococcal group B meningococcal outer membrane protein complex (OMPC) in the otitis media (OM) model in squirrels, and the squirrel pair Homologs and heterotypic attacks.A total of 70 squirrels aged 1-2 years old and weighing 400-600 g were randomly divided into 8 groups, 34 inoculated with vaccine and 36 inoculated with placebo.The vaccine consisted of 6B, 14, 19F and 23F pneumococci PS and OMPC coupling squirrel inoculated at 0 and 4 weeks, two doses of vaccine, the dose of 2μgPS / kg 8 weeks after the first dose of immunization, respectively, 0.1ml of 6A, 6B, 19A or 19F pneumococcal suspension (200-370 CFU / ml) were injected into the medial otolith space of the squirrel for challenge and 3, 7 and 14 days after the surgery for otoscopy and tympanometry and middle ear aspiration. Each immunization Cardiac puncture was performed before (day 0 and week 4), before (week 8) and after 2 weeks (week 10), and anti-6A, 6B, 19A and 19F PS-specific IgG antibodies were detected by ELISA. The results showed that anti-PS-PS antibodies were not detected in pre-immunized squirrel serum.The PS-specific antibodies against 6A, 6B, 19A and 19F in vaccine group were significantly increased after immunization, but no significant changes were observed in placebo group. In 6B or 6A type bacteria