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[目的]探讨检测PTEN,LKB1基因缺失、突变对鉴别良恶性胸腔积液的价值.[方法]提取漏出液组43例、良性胸腔积液组44例、恶性胸腔积液组47例患者胸腔积液中的DNA,采用PCR-SSCP法检测PTEN第5~8外显子、LKB1第1~9外显子缺失、突变情况.[结果]与漏出液组比较,良性胸腔积液组未发现PTEN,LKB1基因缺失或突变;恶性胸腔积液组PTEN基因总改变率为46.8%,LKB1基因总改变率为42.6%,联合检测PTEN,LKB1诊断恶性胸腔积液的灵敏度为78.7%,显著高于胸腔积液细胞学检查的53.2%(P<0.05).[结论]PCR-SSCP技术检测PTEN,LKB1基因缺失、突变可作为鉴别良恶性胸腔积液的辅助方法.
[Objective] To explore the value of detecting the deletion and mutation of PTEN and LKB1 gene in differential diagnosis of benign and malignant pleural effusions. [Methods] Forty-three patients with leaking fluid, 44 patients with benign pleural effusion, 47 patients with malignant pleural effusion PCR-SSCP method was used to detect the deletion and mutation of exons 5-8 of PTEN and exon 1- 9 of LKB1.Results Compared with the leakage group, no PTEN was found in benign pleural effusion group , LKB1 gene deletion or mutation. The total change rate of PTEN gene in malignant pleural effusion group was 46.8% and the total change rate of LKB1 gene was 42.6%. The sensitivity of combined detection of PTEN and LKB1 in the diagnosis of malignant pleural effusion was 78.7% (P <0.05). [Conclusion] The detection of PTEN and LKB1 gene deletion by PCR-SSCP can be used as an auxiliary method to distinguish benign and malignant pleural effusions.