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收集婴幼儿急疹及淋巴系统增生性疾病患者外周血单个核细胞进行体外培养，从７例婴幼儿急疹及２例淋巴系统增生性疾病患者中分离出一种病毒，此病毒能在ＰＨＡ激活的人脐血单个核细胞中传代生长，产生典型ＣＰＥ：形成气球样巨细胞。电镜下观察，感染细胞中可见直径１８０ｎｍ左右，有包膜，疱疹样病毒颗粒；血清学试验证明分离株与ＨＳＶ－１，２、ＨＣＭＶ、及ＥＢＶ无抗原交叉，而与ＨＨＶ－６ＧＳ株间存在抗原一致性；多聚酶链反应表明该分离株ＨＨＶ－６特异性ＤＮＡ阳性；综合以上结果，初步认为该分离株为ＨＨＶ－６。同时还用ｐＣＲ法对所收集的标本直接检测ＨＨＶ－６特异性ＤＮＡ。ＰＣＲ法与病毒分离法相比较，前者ＨＨＶ－６检出率为８８．８％（１６／１８）．后者为３８．９％（７／１８）。
Peripheral blood mononuclear cells from infants and young children with acute rash and lymphatic system proliferative diseases were collected and cultured in vitro. A virus was isolated from 7 infants and young children with acute rash and 2 patients with lymphatic system proliferative diseases. The virus could be activated in PHA Of human umbilical cord blood mononuclear cells subculture, resulting in a typical CPE: the formation of balloon-like giant cells. Electron microscopy showed that the infected cells were found to be around 180 nm in diameter with enveloped and herpes-like virus particles. Serological tests showed that the isolates did not cross with HSV-1, 2, HCMV and EBV, but had no cross with HHV-6GS The results of polymerase chain reaction (PCR) showed that the specific DNA of HHV-6 was positive. According to the above results, HHV-6 was considered as HHV-6. HGV-6-specific DNA was also directly detected by the pCR method. Compared with the virus isolation method, the detection rate of HHV-6 in the former was 88.8% (16/18). The latter was 38.9% (7/18).