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【目的】构建不同程度冻伤的动物模型。【方法】32只大鼠随机分为空白对照组、Ⅰ度高原冻伤组、Ⅱ度高原冻伤组、Ⅲ度高原冻伤组，每组8只，应用大型复合低压舱模拟高原环境，大鼠缺氧习服2周后，通过控制液氮直接冷冻时间构建Ⅰ~Ⅲ度高原冻伤模型，对照组不处理。各组建模48 h 后，采用静脉注射Sovovue 进行超声造影，对冻伤部位血液微循环成像；并对各组大鼠冻伤部位组织进行 HE 染色，观察不同程度冻伤组织病理学变化。【结果】三组不同程度高原冻伤组织随冻伤程度的增加血流阻滞呈明显加重趋势(P <0.05)；Ⅰ度高原冻伤组织表皮层损伤，有红细胞渗出；Ⅱ度高原冻伤损伤至真皮深层和肌肉表层；Ⅲ度高原冻伤破坏了几乎全部肌肉层(P <0.05)。【结论】通过控制液氮直接冷冻时间能成功构建Ⅰ~Ⅲ度高原冻伤模型，可操作性强。“,”Objective]To construct the animal plateau frostbite (PF)models .[Methods]32 rats were randomly divided into in blank control group,Ⅰ plateau frostbite group,Ⅱ plateau frostbite group and Ⅲplateau frostbite group,8 rats in each group.A low-pressure composite cabin was used to simulate plateau en-vironment .After 2 weeks of hypoxia in rats,I to III degree plateau frostbite models were constructed by con-trolling the liquid nitrogen freezing time,and the control group was not treated.48 h after modeling in each group,ultrasonic imaging was conducted by the intravenous Sovovue on the frozen part of the body for blood microcirculation imaging,and the frostbite parts of tissues of rats in each group were stained by HE staining. The pathological changes of different degree frostbitten tissue were observed.[Results]Blocked blood flow of the three groups was significantly increased with the increased degree of frostbite on plateau frozen tissue (P<0.05);Ⅰ plateau frozen tissue shew epidermis injury with leakage of red blood cell;Ⅱ plateau frostbite in-jury was suffered to the deep dermis and muscle surface;Ⅲ degree of plateau frostbite destroyed the almost all the muscle layer (P <0.05).[Conclusion]Ⅰ~Ⅲ plateau frostbite models can be directly constructed by con-trolling the liquid nitrogen freezing time .This approach has strong operability.