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目的探讨玻璃化冷冻对卵裂期胚胎发育潜能的影响。方法对40例经徐州市妇幼保健院生殖中心实施玻璃化冷冻卵裂期胚胎(玻璃化冷冻组)和41例程序冷冻卵裂期胚胎(对照组)行复苏移植术作为对照,比较两组的复苏胚胎存活率、种植率、临床妊娠率。结果在玻璃化冷冻组的40例中,共复苏胚胎81个,存活胚胎77个,复苏率95.1%,共移植79个胚胎,种植率为30.4%,临床妊娠率为50%;对照组共41例,解冻135个胚胎,复苏后存活87个胚胎,存活率64.4%,种植率为17.9%,临床妊娠率为26.8%。玻璃化冷冻组的胚胎存活率、种植率、妊娠率均显著高于对照组(P<0.05)。结论玻璃化冷冻和程序冷冻均适于人卵裂期胚胎的冷冻,但玻璃化冷冻法更好的保存了胚胎的发育潜能,能够获得更好的冷冻效率。
Objective To investigate the effect of vitrification on the developmental potential of cleavage stage embryos. Methods Forty cases of vitrified frozen cleavage stage embryos (vitrification group) and 41 cases of frozen stage cleavage stage embryos (control group) underwent reproductive transplantation in the Reproductive Center of Xuzhou MCH center as control Resuscitation embryo survival rate, implantation rate, clinical pregnancy rate. Results In 40 cases of vitrification group, 81 embryos were resuscitated and 77 survived, the recovery rate was 95.1%. A total of 79 embryos were transplanted. The implantation rate was 30.4% and the clinical pregnancy rate was 50%. The control group had 41 For example, 135 embryos were thawed and 87 survived after resuscitation. The survival rate was 64.4%, the implantation rate was 17.9% and the clinical pregnancy rate was 26.8%. Vitrification freezing group embryos survival rate, implantation rate, pregnancy rate were significantly higher than the control group (P <0.05). Conclusion Both vitrification and cryopreservation are suitable for cryopreservation of human cleavage stage embryos. However, vitrification method can better preserve embryonic development potential and achieve better freezing efficiency.