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目的:检测结缔组织生长因子(CTGF)在小鼠肺内的表达,并探讨其在肺纤维化中的意义。方法:通过向气管内滴入博莱霉素建立小鼠肺纤维化模型。用免疫组化染色方法检测不同时期肺组织中CTGF蛋白的表达;用RT PCR检测CTGFmRNA的表达;用酸水解法测定肺羟脯氨酸的含量。结果:在正常小鼠肺组织中,CTGF蛋白和其mRNA几乎不表达;而在模型组的肺组织中表达明显升高(P<0.01)。CTGF蛋白表达于支气管/细支气管上皮细胞、肺泡Ⅱ型上皮细胞和肺成纤维细胞,表达量与肺组织中羟脯氨酸的含量呈显著的正相关(r=0.92,P<0.001)。结论:CTGF与肺纤维化的形成关系密切,CTGF检测可作为评价肺纤维化发生、发展的一种灵敏的指标。
Objective: To detect the expression of connective tissue growth factor (CTGF) in the lungs of mice and to explore its significance in pulmonary fibrosis. Methods: Mouse model of pulmonary fibrosis was established by intratracheal instillation of bleomycin. The expression of CTGF protein in lung tissue was detected by immunohistochemical staining at different time points. The expression of CTGF mRNA was detected by RT PCR. The content of lung hydroxyproline was determined by acid hydrolysis. Results: CTGF protein and its mRNA were almost not expressed in the normal mouse lung tissue, but significantly increased in the lung tissue of the model group (P <0.01). CTGF protein expression in bronchial / bronchial epithelial cells, alveolar type II epithelial cells and lung fibroblasts, the expression of hydroxyproline content in lung tissue was positively correlated (r = 0.92, P <0.001). Conclusion: CTGF is closely related to the formation of pulmonary fibrosis. CTGF can be used as a sensitive index to evaluate the occurrence and development of pulmonary fibrosis.