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目的观察大豆异黄酮对老龄雌性大鼠卵巢及血清中主要性激素水平影响。方法选用20月龄老龄雌性大鼠50只,按体重随机分为5组,每组10只,分别为空白对照组,大豆异黄酮低、中、高剂量组(100、200、300mg/kg)、雌激素组(己烯雌酚0.6 mg/kg),连续给予8周。采用酶联免疫吸附实验测定各组大鼠卵巢及血清中雌二醇、孕酮、睾酮、黄体生成素(LH)、促卵泡生成素(FSH)、性激素结合球蛋白(SHBG)水平。结果与对照组比较,高剂量大豆异黄酮组大鼠卵巢/体比(0.031±0.008)明显降低(P<0.05),血清中雌二醇含量[(5.177±0.453)μg/L]明显升高,LH含量[(3.225±0.405)ng/L]、FSH含量[(1.536±0.186)IU/L]降低(P<0.05);与对照组比较,大豆异黄酮组大鼠血清中孕酮、睾酮、SHBG水平无明显变化(P>0.05)。结论大豆异黄酮可通过调节老龄雌性大鼠血清中雌二醇、LH、FSH水平缓解卵巢功能衰退。
Objective To observe the effects of soy isoflavones on the levels of sex hormones in ovaries and serum of old female rats. Methods Fifty aged 20-month-old female rats were randomly divided into 5 groups according to body weight, with 10 rats in each group as the blank control group, low, middle and high dose of soy isoflavones group (100, 200, 300 mg / kg) , Estrogen group (diethylstibestrol 0.6 mg / kg) for 8 weeks. The levels of estradiol, progesterone, testosterone, luteinizing hormone (LH), follicle stimulating hormone (FSH) and sex hormone binding globulin (SHBG) in ovary and serum of rats in each group were measured by enzyme-linked immunosorbent assay. Results Compared with the control group, the ovary / body ratio (0.031 ± 0.008) and the estradiol level (5.177 ± 0.453) μg / L] in the high-dose soy isoflavone group were significantly lower (P <0.05) , LH content [(3.225 ± 0.405) ng / L] and FSH content [(1.536 ± 0.186) IU / L] were significantly lower than those in the control group , SHBG levels did not change significantly (P> 0.05). Conclusion Soy isoflavones can relieve ovarian dysfunction by regulating estradiol, LH and FSH levels in the serum of aged female rats.