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为了采用草本植物以改进柑桔裂皮病类病毒(CEV)鉴定方法,曾进行了若干试验。用若干 CEV 强株系作接种源。观察结果如后。1.接种源的制备:从感染的柑桔叶片中用酚提取,加入乙醇沉淀。为了确定更可靠的接种方法,用爪哇三七(Gynura auran-tiaca)或番茄“Rutger”品种做摩擦切割试验。摩擦接种是用金钢砂撒在叶片上,用棉球洗擦。用迟钝刀片做切割接种。对茎部进行20次切割就能使 CEV 在爪哇三七植株上有
In order to use herbs to improve the identification of citrus splitvirus (CEV), several tests have been conducted. Several CEV strains were used as inoculation sources. Observed results as follows. 1. Preparation of Inoculum: Extraction of phenol from infected citrus leaves with ethanol precipitation. In order to determine a more reliable method of inoculation, a friction cut test was conducted with Gynura aurattiac or tomato “Rutger” varieties. Frictional inoculation is sprinkled with gold sand on the leaves, with a cotton ball scrub. Cut with a dull blade for vaccination. CEV can be found on Panax notoginseng plants by cutting the stem 20 times