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目的:通过比较不同溶剂萃取青蒿的粗提物对结肠癌HT-29和LoVo细胞的核因子κB(NF-κB)活性的影响,寻找青蒿中对结肠癌细胞NF-κB活性有作用的萃取相。方法:分别用石油醚、氯仿、乙酸乙酯、正丁醇对青蒿水煎液进行萃取,并将萃取得到的粗提物分别处理结肠癌HT-29细胞和LoVo细胞,提取核蛋白后采用凝胶阻滞分析(EMSA)方法对NF-κB活性进行测定。结果:青蒿水煎液氯仿萃取相对HT-29细胞和LoVo细胞的NF-κB活性表达均有明显的抑制作用(P<0.05),其它各萃取相对两种细胞NF-κB活性的影响与对照组比较无明显差异。结论:在不同溶剂萃取青蒿水煎液中,氯仿萃取相粗提物对结肠癌HT-29细胞和LoVo细胞的NF-κB活性具有抑制作用。
OBJECTIVE: To investigate the effects of different extracts of Artemisia annua L. on the activity of nuclear factor-kappa B (NF-κB) in colon cancer HT-29 and LoVo cells, and to find the effect of Artemisia annua on the activity of NF-κB in colon cancer cells Extraction phase. Methods: Artemisia annua decoction was extracted with petroleum ether, chloroform, ethyl acetate and n-butanol, respectively. The extracted crude extracts were used to treat colon cancer HT-29 cells and LoVo cells respectively. The activity of NF-κB was measured by gel-block analysis (EMSA). Results: The chloroform extraction of A. annua decoction significantly inhibited the activity of NF-κB in HT-29 cells and LoVo cells (P <0.05), and the effects of other extracts on NF-κB activity in both cells were similar to those in control No significant difference between groups. CONCLUSION: Crude extracts of chloroform extract of Artemisia annua decoction can inhibit the activity of NF-κB in HT-29 cells and LoVo cells.