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目的 探讨山西省吸毒人群中HCV基因型分布现状和基因特征。方法 采集太原强制戒毒所576份吸毒人员血清标本,用ELISA法进行抗HCV的检测;检出抗HCV阳性的血清进一步用型特异性引物RT-巢式PCR法进行基因分型,选择1b型和2a型各一株进行PCR产物直接测序。结果 576份吸毒人员的抗HCV检出率为7.5％其中本省的抗HCV检出率为5.7％,外省为27.7％,外省吸毒者的抗HCV检出率非常显著高于山西省吸毒者(X~2=30.32,P<0.01)。山西省吸毒人群的HCV基因型以1b型为主(78.9%),其次为2a型(15.8%)和1b/2a混合型(5.3％);未检出1a型、2b型和3a型,但山西省分型阳性者中口吸者居多(84.2％)。山西吸毒者HCV 1b型分离株在其核心区的144个核苷酸序列中,与日本、河北、上海、湖南及邻省的同源性都在94％以上,与湖南株的同源性最好(97％);而推导的氨基酸序列与日本的差异最大(7％)。山西吸毒者HCV 2a型分离株在其核心区的174个cD-NA核苷酸序列和推导的氨基酸序列中与日本的同源性分别为94％和96％,但氨基酸的变异小于核苷酸。值得注意的是,nt 522和 nt 564(核心区第209和223位)的变异是所有中国1b型株的共同特征。结论 山西省吸毒人群中HCV基因型以1b为主。在同一亚型中,山西吸毒者的HCV分离株在核心区部分序列与日本及国内其他地区的分?
Objective To investigate the distribution and gene characteristics of HCV genotypes among drug addicts in Shanxi Province. Methods Serum samples from 576 drug addicts in Taiyuan compulsory detoxification center were collected and tested for anti-HCV by ELISA. Genotypes were further detected by RT-nested PCR using anti-HCV positive sera, and type 1b and 2a each type of PCR products for direct sequencing. Results The detection rate of anti-HCV in 576 drug addicts was 7.5%, of which the anti-HCV detection rate was 5.7% in the province and 27.7% in other provinces. The detection rate of anti-HCV in drug addicts in other provinces was significantly higher than that in Shanxi Province ~ 2 = 30.32, P <0.01). HCV genotypes in Shanxi province were predominantly type 1b (78.9%), followed by type 2a (15.8%) and 1b / 2a (5.3%); type 1a, type 2b and type 3a were not detected Shanxi Province, the majority of mouth-sucking inhalers (84.2%). The HCV 1b isolates from Shanxi drug addicts shared more than 94% homology with the isolates of Japan, Hebei, Shanghai, Hunan and neighboring provinces in the 144 nucleotides of their core region, Good (97%); while the deduced amino acid sequence with Japan the largest difference (7%). The HCV 2a isolates from Shanxi drug addicts shared 94% and 96% homology with Japan respectively in 174 cD-NA nucleotide sequences and deduced amino acid sequences in their core regions, but their amino acid variation was smaller than that of nucleotides . It is noteworthy that the variation of nt 522 and nt 564 (nucleotides 209 and 223 in the core region) is a common feature of all Chinese type 1b strains. Conclusion The genotypes of HCV in Shanxi drug users are 1b. In the same subtype, Shanxi drug addicts HCV isolates in the core part of the sequence with Japan and other parts of the country?