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用两个籼稻品种Ｈ３５９和Ａｃｃ８５５８杂交衍生的重组自交系（ＲＩ，Ｆ７代，１３１个系）群体，构建了一个密度较高、分子标记分布较均匀的分子连锁图。该图全距１３８０．１ｃＭ，包括１４７个ＲＦＬＰ和７８个ＡＦＬＰ标记，标记间的平均距离为６．１ｃＭ。在ＡＦＬＰ分析中，采用５个ＰｓｔＩ／ＭｓｅＩ的引物组合，总共产生了７８个ＡＦＬＰ标记，分布在所有的１２条染色体上，平均每个引物组合产生１５．６个ＡＦＬＰ标记，表明ＡＦＬＰ是一种高效的分子标记，可与ＲＦＬＰ标记结合，用于遗传连锁图谱的构建
A molecular linkage map with higher density and more uniform molecular markers was constructed by using recombinant inbred lines (RI, F7 and 131 lines) derived from two indica hybrids H359 and Acc8558. The map was 1380.1 cM at full range, including 147 RFLPs and 78 AFLP markers with an average distance of 6.1 cM between markers. In the AFLP analysis, a total of 78 AFLP markers were generated using a combination of five PstI / Msel primers, which was distributed across all 12 chromosomes and resulted in an average of 15.6 AFLP markers per primer combination, indicating that AFLP is a Efficient molecular markers can be combined with RFLP markers for the construction of genetic linkage maps