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黑土中土著大豆根瘤菌和不同大豆栽培品种共生固氮活性,在大豆苗期较低,逐渐增加,到结荚鼓粒期达高峰,固氮活性一般为C_2H_46—10μmole株~(-1)小时~(-1),最高C_2H_442μmole株~(-1)小时~(-1)。在不同大豆栽培品种上的结瘤数也是苗期较少,逐渐增加,到鼓粒期达最多,根瘤数目的增加,主要是侧根上根瘤数目的增加。固氮量初测有低肥地比高肥地为高的趋向,高肥地57.1～125.2公斤/公顷,占大豆所需全氮的48～67％,低肥地为66.8～151公斤/公顷,占71～85％。大豆植株中全氮的分布结瘤大豆在花荚中占44.8～56.38％,不结瘤大豆占20.8～49.0％。
The symbiotic nitrogen fixation activity of indigenous soybean rhizobia and different soybean cultivars in black soil was lower and increased gradually at seedling stage and reached its peak at the stage of podding and drumming. The nitrogen fixation activity was generally C 2 H_ 46-10μmole ~ (-1) h ~ (-1) -1), the highest C_2H_442μmole strain ~ (-1) hours -1. The number of nodules on different soybean cultivars was also less in the seedling stage and gradually increased to the maximum in the drum stage with an increase in the number of nodules, mainly the increase in the number of nodules on the lateral roots. The initial nitrogen fixation test showed the trend of low-fat soil and high-fat soil was high, with high-fat land ranging from 57.1 to 125.2 kg / ha, accounting for 48-67% of the total nitrogen required for soybean and 66.8-151 kg / ha for low-fat land, Accounting for 71 ~ 85%. Distribution of total nitrogen in soybean plants The nodulated soybean accounted for 44.8- 56.38% in flower pods and non-nodulated soybean accounted for 20.8-49.0%.