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为了确定污染肝素钠中污染物多硫酸软骨素的来源,将肝素钠的主要原料猪小肠分离为肠粘膜、浆膜、肠衣,采用酶解法提取其中的粘多糖成分,并采用1 HNMR对提取的粘多糖进行定性、定量分析。结果表明,肠粘膜的粘多糖主要成分为肝素钠,并含有硫酸皮肤素和硫酸软骨素C;浆膜中肝素钠和硫酸皮肤素的含量相当,并含有少量硫酸软骨素C;肠衣的粘多糖主要成分为硫酸皮肤素,并含有肝素钠、硫酸软骨素A及硫酸软骨素C;肠粘膜、浆膜、肠衣均不含多硫酸软骨素。
In order to determine the source of contaminating chondroitin sulfate contaminated heparin sodium, the main raw material of heparin sodium was separated into intestinal mucosa, serosal and enteric coat, and the mucopolysaccharide was extracted by enzymolysis method and extracted by 1 H NMR Mucopolysaccharides qualitative, quantitative analysis. The results showed that the main component of mucopolysaccharide is heparin sodium, and contains dermatan sulfate and chondroitin sulfate C; the content of heparin sodium and dermatan sulfate in the serosal membrane is similar, and contains a small amount of chondroitin sulfate C; mucopolysaccharide The main components of dermatan sulfate, and contains heparin sodium, chondroitin sulfate A and chondroitin sulfate C; intestinal mucosa, serosa, casings are not containing chondroitin sulfate.