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目的探讨重组腺病毒介导的mda-7/IL-24基因联合阿霉素(ADM)治疗裸鼠肝癌的作用及机制。方法采用mda-7/IL-24的重组腺病毒载体(Ad-mda-7)和/或ADM治疗实验性肝癌裸鼠,观察各组裸鼠生长时间及肿瘤体积变化,并对各组瘤体组织进行TUNEL检测。结果成功构建了重组腺病毒mda-7/IL-24基因载体。Ad-mda-7+ADM联合治疗组裸鼠平均生存时间为(83.8±4.82)d,明显长于其他3组(P<0.01);Ad-mda-7+ADM组肝癌体积明显缩小,抑癌率为79.78%,与单独用药组和对照组比较差异有统计学意义(P<0.05);联合组癌组织凋亡增加,凋亡指数为38.1%±4.2%,与其余3组比较差异有统计学意义(P<0.05)。结论重组腺病毒介导mda-7/IL-24联合阿霉素有明显的协同抗肿瘤作用,效果优于单独治疗组,主要作用机制与促进癌组织凋亡有关。
Objective To investigate the effect and mechanism of recombinant adenovirus mediated mda-7 / IL-24 gene combined with adriamycin (ADM) on hepatocellular carcinoma in nude mice. Methods Experimental hepatocellular carcinoma (HCC) nude mice were treated with recombinant adenovirus vector (Ad-mda-7) and / or ADM of mda-7 / IL-24 and the growth time and tumor volume of nude mice in each group were observed. TUNEL tissue was tested. Results The recombinant adenovirus mda-7 / IL-24 gene vector was successfully constructed. The mean survival time of the nude mice treated with Ad-mda-7 + ADM was (83.8 ± 4.82) d, significantly longer than that of the other three groups (P <0.01). The volume of HCC was significantly reduced in Ad-mda-7 + (79.78%), which was significantly different from that of the drug alone group and the control group (P <0.05). The apoptosis rate of the combined group was 38.1% ± 4.2%, which was statistically different from the other three groups Significance (P <0.05). Conclusion The recombinant adenovirus mediated mda-7 / IL-24 combined with doxorubicin has obvious synergistic antitumor effect, and its effect is better than that of the monotherapy group. The main mechanism is related to promoting the apoptosis of cancer tissue.