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目的探讨Musashi2(Msi2)对白血病K562细胞体外侵袭能力的影响。方法将靶向Msi2基因的si-RNA1、siRNA2及阴性对照序列转染K562细胞,同时设空白对照组(未转染的K562细胞)。实时荧光定量PCR法及Western blot法分别检测K562细胞中Msi2基因m RNA转录及蛋白的表达水平;经细胞生长曲线观察细胞体外增殖能力;细胞黏附试验检测K562细胞体外黏附能力;Transwell试验检测K562细胞体外迁移及侵袭能力。结果与阴性对照组及空白对照组比较,Msi2-1及Msi2-2组K562细胞中的Msi2基因m RNA转录及蛋白的表达水平显著下降(P<0.05),体外黏附、迁移、侵袭能力明显减弱(P<0.05)。结论抑制Msi2的表达可显著抑制白血病K562细胞的体外侵袭能力,本实验为进一步研究Msi2在白血病发生发展中的调控机制奠定了基础。
Objective To investigate the effect of Musashi2 (Msi2) on the invasion of leukemia K562 cells in vitro. Methods K562 cells were transfected with si-RNA1, siRNA2 and negative control sequences targeting Msi2 gene. Meanwhile, a blank control group (untransfected K562 cells) was established. Real-time PCR and Western blot were used to detect the mRNA and protein expression levels of Msi2 gene in K562 cells respectively. The proliferation of K562 cells was observed by cell growth curve. The adhesion ability of K562 cells in vitro was detected by cell adhesion assay. In vitro migration and invasion ability. Results Compared with the negative control group and the blank control group, Msi2-1 and Msi2-2 group Msi2 gene m RNA transcription and protein expression decreased significantly (P <0.05), in vitro adhesion, migration, invasion was significantly reduced (P <0.05). Conclusion Inhibition of Msi2 expression can significantly inhibit the in vitro invasion of leukemia K562 cells. This study lays the foundation for further study on the regulatory mechanism of Msi2 in the development of leukemia.