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目的 :探讨术中输血对喉癌患者细胞免疫功能的影响。方法 :利用 EPICS- XL 型流式细胞仪对 36例喉癌手术患者于术前及术后 2周进行 T细胞亚群、NK细胞和 CD2 8测定 ,并与正常人及术中输异体血和非输血者进行比较。结果 :136例喉癌患者术前 CD3、CD4、NK细胞、CD2 8下降 ,与对照组比较 ,其差异均有显著性意义 (P<0 .0 1和 0 .0 5 )。 2输异体血组输血后 CD3、CD4、NK细胞、CD2 8下降 ,与治疗前喉癌组比较 ,其差异亦有显著性意义 (均 P <0 .0 5 )。 3非输血组术后 CD3、CD4、CD8、NK细胞、CD2 8与治疗前喉癌组比较 ,其差异均无显著性意义(均 P >0 .0 5 )。结论 :喉癌患者的细胞免疫功能普遍低下 ,术中输异体血会使患者的免疫功能进一步降低 ,使喉癌易于扩散与转移 ,应引起高度重视。
Objective: To investigate the effect of intraoperative blood transfusion on cellular immune function in patients with laryngeal cancer. Methods: T cell subsets, NK cells and CD28 were measured in 36 cases of laryngeal cancer patients before and 2 weeks after operation by EPICS-XL flow cytometry. The results were compared with those of normal and intraoperative blood transfusion Non-blood transfusion were compared. Results: The preoperative CD3, CD4, NK cells and CD28 of 136 laryngeal cancer patients were significantly lower than those of the control group (P <0.01 and 0.05). 2 transfusion blood transfusions CD3, CD4, NK cells, CD28 decreased, compared with pre-treatment laryngeal group, the difference was also significant (P <0.05). There was no significant difference in CD3, CD4, CD8, NK cells and CD8 before and after treatment in non-blood transfusion group (all P> 0.05). Conclusion: The cellular immune function of patients with laryngeal carcinoma is generally low. Intraoperative blood transfusion will further reduce the immune function of patients, so that laryngeal carcinoma is easy to spread and metastasis, which should be given high priority.