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目的：建立测定血浆和脑脊液中甲氨蝶呤的反相高效液相色谱方法。方法：采用ＯＤＳ不锈钢柱，流动相：甲醇∶０．０２５ｍｏｌ·Ｌ－１磷酸盐缓冲液（２１∶７９，ｐＨ６．７０），检测波长：３０６ｎｍ，样品经沉淀蛋白质后直接进样。结果：血浆和脑脊液样品回收率分别为９７．５％±４．１％和１００．８％±１．５％，日内和日间误差（ＲＳＤ）均＜７％，最低检测浓度４．７５×１０－８ｍｏｌ·Ｌ－１，血浆中在１．１０×１０－７～２．２×１０－６ｍｏｌ·Ｌ－１和２．２０×１０－６～１．７６×１０－４ｍｏｌ·Ｌ－１；脑脊液中在１．１０×１０－７～２．２０×１０－６ｍｏｌ·Ｌ－１和２．２０×１０－６～１．１０×１０－３ｍｏｌ·Ｌ－１浓度范围内有良好线性关系，ｒ＝０．９９９６～０．９９９９。结论：本法简便、准确、灵敏。
Objective: To establish a method for the determination of methotrexate in plasma and cerebrospinal fluid by reversed-phase high performance liquid chromatography. Methods: ODS stainless steel column was used. The mobile phase was methanol: 0.025 mol·L-1 phosphate buffer (21:79, pH 6.70). The detection wavelength was 306 nm. The sample was directly injected after precipitation of protein. Results: The recovery rates of plasma and cerebrospinal fluid samples were 97.5% ± 4.1% and 100.8% ± 1.5%, respectively. The intra-and inter-day errors (RSDs) were <7% and the lowest detection concentration was 4.75 × 10-8mol·L-1 in plasma and 1.10 × 10-7 ~ 2.2 × 10-6mol·L-1 and 2.20 × 10-6 ~ 1.76 × 10-4mol·L-1 in plasma ; There was a good linear relationship between CSF concentration of 1.10 × 10-7 ~ 2.20 × 10-6 mol·L-1 and 2.20 × 10-6 ~ 1.10 × 10-3 mol·L-1 , R = 0.9996 ~ 0.9999. Conclusion: This method is simple, accurate and sensitive.