目的建立稳定表达A类I型清道夫受体真核表达载体的人胚肾细胞——HEK293细胞系。方法构建pRc/CMV-A类I型清道夫受体真核表达载体,应用脂质体介导的转染技术将该质粒导入HEK293细胞,再用G418筛选稳定的细胞表达系。真核细胞中A类I型清道夫受体的表达分别用逆转录聚合酶链反应与W Objective To establish a human embryonic kidney cell line HEK293 stably expressing a class I scavenger receptor eukaryotic expression vector. Methods The eukaryotic expression vector pRc / CMV-A type I scavenger receptor was constructed and transfected into HEK293 cells by liposome-mediated transfection. The stable cell expression lines were screened by G418. A type I scavenger receptor expression in eukaryotic cells were reverse transcribed polymerase chain reaction and W