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Objective To investigate the role of caspase 3 and its inhibitor Ac DEVD CHO in rat lens epithelial cell apoptosis induced by hydrogen peroxide (H 2O 2) in vitro Methods Rat lenses were incubated in modified Eagle’s medium containing 2 mmol/L H 2O 2 to induce apoptosis in vitro Apoptosis in lens epithelial cells was assessed by transmission electron microscopy and annexin V propidium iodide (PI) double staining flow cytometry after 12, 24 and 48 h of incubation The activity of caspase 3 was analyzed by western blotting Results Observations under transmission electron microscopy revealed that 2 mmol/L H 2O 2 could effectively induce lens epithelial cell apoptosis in vitro Caspase 3 activity increased during cell apoptosis and the peak measurement occurred at 24 h after treatment with H 2O 2 Cell apoptosis was blocked by caspase 3 inhibitor Ac DEVD CHO Conclusions The activation of caspase 3 plays an important role in executing apoptosis in H 2O 2 treated lens epithelial cells and in the formation of cataract The caspase 3 inhibitor Ac DEVD CHO may effectively prevent lens epithelial cell apoptosis caused by oxidative injury
Objective To investigate the role of caspase 3 and its inhibitor Ac DEVD CHO in rat lens epithelial cell apoptosis induced by hydrogen peroxide (H2O2) in vitro Methods Rat lenses were incubated in modified Eagle’s medium containing 2 mmol / LH202 to induce apoptosis in vitro Apoptosis in lens epithelial cells was assessed by transmission electron microscopy and annexin V propidium iodide (PI) double staining flow cytometry after 12, 24 and 48 h of incubation The activity of caspase 3 was analyzed by western blotting Results Observations under transmission electron microscopy revealed that 2 mmol / LH 2 O 2 could have induced lens epithelial cell apoptosis in vitro Caspase 3 activity increased during cell apoptosis and the peak measurement occurred at 24 h after treatment with H 2O 2 Cell apoptosis was blocked by caspase 3 inhibitor Ac DEVD CHO Conclusions The activation of caspase 3 plays an important role in executing apoptosis in H 2O 2 treated lens epithelial cells and in the formation of cataract The caspase 3 inhibitor Ac DEVD CHO may effectively prevent lens epithelial cell apoptosis caused by oxidative injury