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用Ｎｏｒｔｈｅｒｎ杂交、免疫细胞化学和电镜技术，以垂体ＡＣＴＨ瘤细胞系（ＡｔＴ２０）为对照，从转录、翻译及亚细胞水平检测了５种垂体外不同病理组织来源的肿瘤细胞系ＡＣＴＨ基因表达，包括属于ＡＰＵＤ系统的两种人小细胞肺癌（ＳＣＬＣ－Ｈ６９和ＳＣＬＣ），３种非ＡＰＵＤ系统肿瘤：肺腺癌（ＡＧＺＹ８３－α、低分化胃粘液癌（ＭＧＣ－８０３）和肝癌（Ｂｅｌ－７４０２）。结果证实，（１）ＡＣＴＨ基因表达不仅存在于ＡＰＵＤ系统肿瘤，而且也存在于非ＡＰＵＤ系统肿瘤，如ＡＧＺＹ８３－α和ＭＧＣ－８０３。（２）不同细胞系中ＰＯＭＣ（ＡＣＴＨ前体）转录的ｍＲＮＡ表达长度不同（８００ｂｐ、１２００ｂｐ、１４００ｂｐ）。支持激素类物质合成在组织中普遍存在的假说，但存在着质的差别
By using Northern blot, immunocytochemistry and electron microscopy techniques, pituitary ACTH tumor cell line (AtT20) was used as a control, and the expression of ACTH genes in tumor cell lines derived from five different pituitary tissues in vitro was examined from the transcription, translation, and subcellular levels. Two human small cell lung cancers (SCLC-H69 and SCLC) of the APUD system, three non-APUD system tumors: lung adenocarcinoma (AGZY83-α, poorly differentiated gastric mucinous carcinoma (MGC-803), and liver cancer (Bel-7402) The results confirm that (1) ACTH gene expression is not only present in APUD system tumors, but also in non-APUD system tumors, such as AGZY83-α and MGC-803. (2) Transcription of POMC (ACTH precursor) in different cell lines Different mRNA expression lengths (800bp, 1200bp, 1400bp) support the hypothesis that hormonal synthesis is ubiquitous in tissues, but there are qualitative differences