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一、校准前的准备质谱要抽到工作所需的真空度,时间比较长,所以需要提前一天通知客户开质谱仪抽真空。抽真空是为了减少离子碰撞损失,保证仪器有高的灵敏度。如果真空度达不到做样要求,禁止尝试做样。因为在低真空度下做样,大量氧分子可能会烧坏离子源灯丝,也可能会引起其他分子离子反应,使质谱图杂乱。流动相也可要求客户提前一天配好,并以较低的流速平衡柱子。如果校准当天现配流动相,超声脱气、平衡柱子就会花费较长时间。对于单四极杆的仪器,
First, the pre-calibration mass spectrometry to be drawn to the vacuum required for work, a long time, so need to inform the customer a day in advance to open the mass spectrometer vacuum. Vacuum is to reduce ion collision losses, to ensure that the instrument has high sensitivity. If the degree of vacuum can not meet the requirement, do not try to do it. Because of the low vacuum done, a large number of oxygen molecules may burn out the ion source filament, may also cause other molecular ion reaction, the spectrum is messy. The mobile phase may also require the customer to be prepared one day in advance and to balance the column at a lower flow rate. If the calibration day is equipped with mobile phase, ultrasonic degassing, the balance of the column will take a long time. For single quadrupole instruments,