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AIM:To evaluate effects of nitric oxide (NO) andperoxynitrite anion (ONOO~-) on lung injury following intestinalischemia-reperfusion (IR) in rats.METHODS:A rat model of intestinal ischemia was madeby clamping superior rnesenteric artery and lung injury wasresulted from reperfusion.The animals were randomlydivided into 3 groups:sham operation (Sham),2 h ischerniafollowed by 2 h reperfusion (IR) and IR pretreated withaminoguanidine (AG) - an inhibitor of inducible NO synthase(iNOS) 15 minutes before reperfusion (IR+AG).The lungrnalondialdehyde (MDA) and nitrate/nitrite (NO_2~-/NO_3~-)contents and morphological changes were examined.Western blot was used to detect the iNOS protein expression.Immunohistochemical staining was used to determine thechange of nitrotyrosine (NT)- a specific“footprint”of ONOO~-.RESULTS:The morphology revealed evidence for lungedema,hemorrhage and polymorphonuclear sequestrationafter intestinal IR.Compared with sham group,lung contentsof MDA and NO_2~-/NO_3~- in IR group were significantly increased(12.00±2.18 vs 23.44±1.25 and 76.39±6.08 vs 140.40±4.34,P<0.01) and the positive signals of iNOS and NT were alsoincreased in the lung.Compared with IR group,the contentsof MDA and NO_2~-/NO_3~- in IR+AG group were significantlydecreased (23.44±1.25 vs 14.66±1.66 and 140.40±4.34 vs80.00±8.56,P<0.01) and NT staining was also decreased.CONCLUSION:Intestinal IR increases NO and ONOOproduction in the lung,which may be involved in intestinalIR-mediated lung injury.
AIM: To evaluate the effects of nitric oxide (NO) andperoxynitrite anion (ONOO ~ -) on lung injury following intestinal ischemia-reperfusion (IR) in rats. METHODS: A rat model of intestinal ischemia was madeby clamping superior rnesenteric artery and lung injury wasresulted from reperfusion. The animals were randomly divided into 3 groups: sham operation (Sham), 2 h ischerniafollowed by 2 h reperfusion (IR) and IR pretreated withaminoguanidine (AG) - an inhibitor of inducible NO synthase (iNOS) (MDA) and nitrate / nitrite (NO 2 - - / NO 3 - -) contents and morphological changes were tested. Western blot was used to detect the iNOS protein expression. Immunohistochemical staining was used to determine the change of nitrotyrosine ) - a specific “footprint” of ONOO ~ -RESRESULTS: The morphology revealed evidence for lungedema, hemorrhage and polymorphonuclear sequestrationafter intestinal IR. Compared with sham group, lung contents of MDA and NO_2 ~ - / NO_3 ~ - i n IR group were significantly increased (12.00 ± 2.18 vs 23.44 ± 1.25 and 76.39 ± 6.08 vs 140.40 ± 4.34, P <0.01) and the positive signals of iNOS and NT were also added in the lung. Compared with IR group, the contents of MDA and NO_2 ~ - / NO_3 ~ - in IR + AG group were significantly decreased (23.44 ± 1.25 vs 14.66 ± 1.66 and 140.40 ± 4.34 vs80.00 ± 8.56, P <0.01) and NT staining was also decreased.CONCLUSION: Intestinal IR increases NO and ONOOproduction in the lung, which may be involved in intestinal IR-mediated lung injury.