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目的 探讨褪黑素增补于停搏液中对缺血再灌注离体鼠心的保护作用。方法 将 2 4只 Wistar大鼠随机分为褪黑素组 (A )和对照组 (B)。离体鼠心在改良的 L angendorff- Neely灌注模型上 30分钟预灌注 ,12 0分钟停搏 ,30分钟再灌注。缺血前及再灌注期间测定血流动力学指标、心肌酶 (包括 CPK、L DH)、心肌超氧化物歧化酶 (SOD)、过氧化脂质 (L PO)含量。电镜观察心肌超微结构。结果 再灌注后 ,A组心功能、心肌超微结构的改善明显优于 B组 ;心肌酶 (CPK,L DH)、过氧化脂质 (L PO)含量显著低于 B组 (P<0 .0 1) ;心肌超氧化物歧化酶 (SOD)含量显著高于 B组 (P<0 .0 1)。结论 褪黑素增补于停搏液中可显著减轻心肌缺血再灌注损伤 ,具有良好的心肌保护作用
Objective To investigate the protective effect of melatonin added to cardioplegic solution on isolated rat heart during ischemia-reperfusion. Methods Twenty-four Wistar rats were randomly divided into melatonin group (A) and control group (B). Ex vivo rat hearts were preperfused for 30 minutes on a modified Langendorff-Neely perfusion model, arrested at 12 minutes and reperfused for 30 minutes. The indexes of hemodynamics, myocardial enzymes (including CPK, L DH), myocardial SOD, LOP were measured before and during reperfusion. Electron microscopy myocardial ultrastructure. Results After reperfusion, the improvement of cardiac function and myocardial ultrastructure in group A was better than that in group B. The contents of CPK, L DH and L PO were significantly lower than those in group B (P <0. 0 1). The content of myocardial SOD was significantly higher than that in group B (P <0.01). Conclusion Melatonin supplementation in cardioplegia can significantly reduce myocardial ischemia-reperfusion injury, has a good myocardial protective effect