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为研究丙戊酸钠对脑组织不同区域γ－氨基丁酸（ＧＡＢＡ）生成的机制，分别给大鼠ｉｐ２００ｍｇ／ｋｇ丙戊酸钠、２００ｍｇ／ｋｇ丙戊酸钠＋１００ｍｇ／ｋｇ氨基羟乙酸（ＡＯＡＡ），３０ｍｉｎ后断头处死，观察下丘脑、小脑、延髓、大脑皮层四个脑区谷氨酸脱羧酶（ＧＡＤ）、γ－氨基丁酸转氨酶（ＧＡＢＡ－Ｔ）活性及ＧＡＢＡ含量的变化。结果表明，丙戊酸钠既抑制ＧＡＢＡ－Ｔ也能激活ＧＡＤ，显著提高下丘脑、大脑皮层两个脑区的ＧＡＢＡ含量。虽然四个脑区的ＧＡＢＡ－Ｔ活性得到显著的抑制，但ＧＡＢＡ并没有相应地显著提高；仅在ＧＡＤ活性显著升高的下丘脑及大脑皮层两个脑区的ＧＡＢＡ含量显著增加。ＡＯＡＡ加丙戊酸钠组与单独ＡＯＡＡ组比较，各脑区ＧＡＢＡ－Ｔ活性无明显差别变化，仅在下丘脑中的ＧＡＤ活性显著提高，相应的ＧＡＢＡ含量也显著增加。提示丙戊酸钠主要是通过激活ＧＡＤ，从而提高脑组织ＧＡＢＡ的含量，这种作用有区域选择性
In order to study the mechanism of sodium valproate on the formation of GABA in different regions of brain tissue, rats were given ip200mg / kg sodium valproate, 200mg / kg sodium valproate + 100mg / kg glyoxylic acid (AOAA ) Were sacrificed after 30 minutes. The changes of glutamate decarboxylase (GAD), GABA-T activity and GABA content in the hypothalamus, cerebellum, medulla oblongata and cerebral cortex were observed. The results showed that sodium valproate not only inhibited GABA-T but also activated GAD, significantly increased the content of GABA in both brain regions of hypothalamus and cerebral cortex. Although GABA-T activity was significantly inhibited in all four brain regions, GABA did not significantly increase correspondingly; GABA levels were significantly increased in both brain regions of the hypothalamus and cerebral cortex, where GAD activity was significantly elevated. Compared with AOAA group alone, there was no significant difference in GABA-T activity between AOAA plus sodium valproate group and AOAA group alone. The GAD activity in the hypothalamus was significantly increased and the corresponding GABA content was also significantly increased. Prompt sodium valproate mainly through the activation of GAD, thereby increasing the content of GABA in brain tissue, this effect has a regional selectivity