迄今大量研究已经证实,肿瘤发生与发展的生物学基础是基因突变,所以,肿瘤基因治疗已成为广大肿瘤学者研究的热点。基因转导和策略选择是肿瘤基因治疗的关键。目前基因转导方法包括DNA-磷酸钙共沉淀、脂质体介导、电穿孔、显微注射、受体介导转移、DNA直接注射、基因枪及病毒介导等,但仍存在许多问题,如基因转移效率低、靶向性不确切、安全性低等。1996年,Toru Nishi等利用电穿孔法转染lacZ基因和单 So far a large number of studies have confirmed that the biological basis of tumorigenesis and development of gene mutations, therefore, gene therapy of tumors has become a hot spot for the majority of oncologists. Gene transduction and strategy selection is the key to cancer gene therapy. Currently, gene transduction methods include DNA-calcium phosphate coprecipitation, liposome-mediated, electroporation, microinjection, receptor mediated transfer, direct DNA injection, gene gun and virus- Such as low efficiency of gene transfer, targeted inaccurate, low safety. In 1996, Toru Nishi et al used electroporation to transfect the lacZ gene and single