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目的 :了解 HBe Ag阴性慢性乙型肝炎 (乙肝 )血清乙肝病毒 (HBV) DNA含量及与临床的关系。方法 :用免疫荧光定量 PCR法检测 35 0例 HBe Ag阴性慢性乙肝 HBV DNA含量。结果 :HBV DNA总阳性率为 5 8% ,临床分型中 HBV携带者阳性率为 2 5 .6 % ,慢性活动性肝炎 (慢活肝 )、肝硬化及肝癌阳性率为 82 .8%～ 10 0 %。前者DNA含量为 10 ( 4.4 2± 1 .35) 拷贝 / m l,后三者为 10 5.2 5～ 10 5.88拷贝 / m l(P <0 .0 1) ,慢活肝又高于肝硬化及肝癌 (P <0 .0 5 ) ,阳性者血清丙氨酸转氨酶 <10 0 U / L者 DNA含量较低 (P <0 .0 5 )。结论 :HBe Ag阴性慢性乙肝多数仍存在病毒活动性复制 ,其病情活动程度与血清 DNA含量有关
Objective: To understand the content of hepatitis B virus (HBV) DNA in HBeAg-negative chronic hepatitis B (HBV) and its clinical significance. Methods: HBV DNA levels in 35 0 HBeAg-negative chronic hepatitis B patients were detected by immunofluorescence quantitative PCR. Results: The positive rate of HBV DNA was 58%, the positive rate of HBV carriers in clinical classification was 25.6%, the positive rate of chronic active hepatitis (chronic active liver), cirrhosis and liver cancer was 82.8% 10 0%. The former DNA content was 10 (4.4 2 ± 1.35) copies / ml, the latter three were 10 5.2 5 ~ 10 5.88 copies / ml (P <0.01), slow and active liver cirrhosis and liver cancer ( P <0.05). The DNA content of serum alanine aminotransferase <10 0 U / L in the positive group was lower (P <0.05). Conclusion: The majority of HBeAg-negative chronic hepatitis B virus still exist active replication, the degree of disease activity and serum DNA content